Cutty Sark is shown as temporarily closed: The station name is struck out, desaturated and with a dagger. The wheelchair symbol has also been removed. 

Bond Street is shown as a walking distance interchange to Oxford Circus from the Elizabeth line blob. Previously there was a single step-free-to-train blob for the Elizabeth and Jubilee lines a short distance northwest of a step-free-to-platform blob on the Central line. Now the Elizabeth line has moved slightly north (increasing the interchange distance to the Central line at TCR in consequence) and the blob moved to a short distance northwest of Oxford Circus. The Central line blob has moved east to be directly southwest of the Elizabeth line blob, meaning the latter is about equidistant to the two Central line markers. The Jubilee line blob is now south west of the Central line blob and is now closer to the Jubilee line marker at Green Park than is to the Elizabeth line at Bond Street. 

Barbican has changed from having an interchange circle to having three ticks (matching Great Portland Street rather than Euston Square)

Leytonstone and Leytonstone High Road are now marked as a walking distance intchange, the latter moves east a bit to accommodate 

Every single station symbol on the DLR from Heron Quays to Lewisham inclusive has moved about a pixel to the right for some reason.

The label for Blackfriars has moved about a pixel south.

In the bottom right of the map frame, the logo has changed from standard London Underground to a silver roundel with a 3D-effect and "TFL 25" on the bar. 

The bottom panel has changed from advertising the new London Overground line names to advertising TfL Go

On the index page:
Barking Riverside: the blue wheelchair symbol has moved from last to first in the sequence.
Cutty Sark: A red dagger replaces the wheelchair symbol
Green Park: Gains a red dagger
Langley: Gains a white wheelchair symbol
Mitcham / Mitcham Eastfields: The double dagger moves from over the "e" of the" "Special" fare zone at Mitcham to it's intended position one line lower at Mitcham Eastfields (a previously blank space)
New Southgate: Moves from the last entry in column 4 to the first in column 5, presumbly in consequence of the change at Seven Sisters
Seven Sisters: The toilet facilities were previously marked as being for the Weaver line only, now they are for the whole station. The station has also gained accessible facilities.
West Hampstead: The Mildmay line station has gained an accessible toilet inside the gateline, the one outside the gateline remains.